ABSTRACT
The present research work was carried out to observe the effect of various concentrations of Benzyl amino purine (BAP) in terms of multiple shoot induction with special emphasis on qualitative and quantitative changes of proteins during multiple shoot formation. From whole embryonated cotyledons of groundnut. The shoot induction medium containing different concentrations of Benzyle amino purine ranged from 5.0 to 25.0mg/l along with lower concentration of Indole acetic acid (IAA).The multiple shoots were observed in all the BAP concentrations at a varying levels. Among the various levels of BAP, 25.0mg/l of BAP plus 0.50mg/l of IAA was found to be the most effective in terms of multiple shoot induction. The growth parameters like plantlet height, fresh and dry weight also highly influenced by the concentration of the BAP. The root induction was achieved in micropropagated shoots by using Indole butyric acid (IBA) at a concentrations of 1.0 to 5.0mg/l. The mean number of roots, root length was highly influenced by IBA. In all the concentrations of IBA the rooting was associated with moderate and heavily associated basal callusing. The quantitative estimation of protein content in regenerated shoots also increased with higher concentrations of BAP. The SDS-PAGE analysis showed that there was a nine visible bands with 82.2, 76.4, 57.5, 27.6, 23.7, 21.4, 18.2, 16.5 and 12.8 kDa were observed in multiple shoots derived from all the concentrations of BAP. Whereas in whole embryonated cotyledonary explants there was a entirely different protein banding
ABSTRACT
Most of the Bartholin's gland abscesses have been thought to be caused by colonizing micro-organisms of the perineal region. We encountered an interesting case of acute Bartholins abscess caused by Streptococcus pneumoniae in a primigravida. The abscess was incised and drained. The patient was treated with Cefuroxime. This case is presented for its rarity.
ABSTRACT
During a one year study 2955 clinical specimens of pus, urine, wound swabs, blood cultures, discharges from fistula, peritoneal aspiration fluid, CSF and sputum were processed to find out the prevalence rate of enterococci. One hundred isolates (3.38%) of Enterococci were obtained which were speciated by conventional methods. The highest incidence was from isolates of urine (43%), pus (40%), wound swabs (11%), and the least incidence was noted in discharges from fistula (2%), blood cultures (2%) and peritoneal aspiration fluid (2%). 98% were Enterococci and among these 88% of the isolates were Enterococcus faecalis. Antibiogram of the isolates were done by Standard disc diffusion method and Vancomycin sensitivity was found to be 95%.
Subject(s)
Enterococcus faecalis/drug effects , Gram-Positive Bacterial Infections/microbiology , Humans , India , Microbial Sensitivity TestsABSTRACT
Two hundred and eighteen bacterial isolates obtained from various clinical samples were subjected to Gram stain by conventional method. For all the isolates potassium hydroxide (KOH) string test and sensitivity to vancomycin were done. Gram positive bacteria showed 100% sensitivity to vancomycin as also 100% negativity for string test. Of the gram negative bacteria, 99.42% were resistant to vancomycin while 98.85% were positive for the string test. KOH and vancomycin tests are simple, inexpensive and can be used in addition to Gram staining for rapid identification of bacterial cultures.